| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Sinorhizobium fredii | ACAGTTGAACGCACGCAG | TGATCGTGGGCAAGAGCAG | 58.99 | 60.08 | 197 | 117 |
100.00%
|
100.00%
|
| Primer 2 | Sinorhizobium fredii | ATCGGCTTCGCATCGGAT | ATGAAGCCGAGCATCCAGG | 59.26 | 59.85 | 167 | 114 |
100.00%
|
100.00%
|
| Primer 3 | Sinorhizobium fredii | GCAGTTCGTTGGCATCCAG | ATCGCCTGTTGGTCGTGT | 59.50 | 59.26 | 279 | 112 |
100.00%
|
100.00%
|
| Primer 4 | Sinorhizobium fredii | ATCGGCTTCGCATCGGAT | CACATTCGCCATGAAGCCG | 59.26 | 59.94 | 177 | 108 |
100.00%
|
100.00%
|
| Primer 5 | Sinorhizobium fredii | ATCGGCTTCGCATCGGAT | CCATGAAGCCGAGCATCCA | 59.26 | 60.15 | 169 | 108 |
100.00%
|
100.00%
|
| Primer 6 | Sinorhizobium fredii | AAAACCGCTGCCATTCGC | TCATCGAAGACTGCACGCA | 59.74 | 59.71 | 290 | 107 |
100.00%
|
100.00%
|
| Primer 7 | Sinorhizobium fredii | ATCGGCTTCGCATCGGAT | TAGGCGTGCATGTGGTGA | 59.26 | 58.94 | 269 | 105 |
100.00%
|
100.00%
|
| Primer 8 | Sinorhizobium fredii | AAAACCGCTGCCATTCGC | CCGACTCTCACCACGTGTT | 59.74 | 59.64 | 186 | 104 |
100.00%
|
100.00%
|
| Primer 9 | Sinorhizobium fredii | TGCAGTTCGTTGGCATCCA | ATCGCCTGTTGGTCGTGT | 60.23 | 59.26 | 280 | 99 |
100.00%
|
100.00%
|
| Primer 10 | Sinorhizobium fredii | AACGCTCGACATCGCCTT | AGAGACCGAGCCACTGGAA | 59.74 | 60.23 | 224 | 98 |
100.00%
|
100.00%
|